clean and sterile experimentRoom (sterile room)

Adapt to the industry:Microbiology, biomedicine, biochemistry, animal experiments, genetic recombination Suggested level: 100-100,000
(One),Sterile laboratory designConstruction
1. Sterile laboratory design
★ The sterile laboratory consists of six parts: preparation room, washing room, sterilization room, sterile room, constant temperature culture room and general laboratory. The common feature of these rooms is that the texture of the floor and walls is smooth and hard, and the instruments and equipment are simply furnished for easy cleaning.

 
2. Basic requirements for sterile laboratory construction
Preparation room
★ The preparation room is used to prepare the culture medium and sample processing. There are counters, test benches, electric stoves, refrigerators, water and sewage pipes, power supplies, etc. for reagent cabinets, storage utensils or materials.
 

Washing room
★ The washing room is used for washing utensils, etc. Pathogenic microorganisms are sometimes present because used utensils have been contaminated with microorganisms. Therefore, it is preferable to provide a washing room when conditions permit. Indoors should be equipped with heaters, steamers, basins and buckets for washing utensils, as well as various bottle brushes, decontamination powder, soap, washing powder, etc.
 

Sterilization room
★ Sterilization room is mainly used for sterilization of culture medium and sterilization of various utensils. Indoors should be equipped with sterilization equipment and facilities such as high pressure steam sterilizer and oven.
 

germ-free room
★ The sterile room, also called the inoculation room, is a specialized laboratory for aseptic operation such as system inoculation and purification of strains. In the microbial work, the inoculation transplantation of the strain is a major operation. The characteristic of this operation is to ensure the pure species of the strain and prevent the contamination of the bacteria. In the air of the general environment, due to the presence of many dust and bacteria, it is easy to cause pollution and interferes greatly with the inoculation work.
 

（two）、Sterilization of sterile rooms
Fumigation: This is a measure of thorough sterilization of the sterile room. The sterile room should be used for a long time. If the pollution is serious, it should be fumigated. It can be fumigated with formaldehyde, lactic acid or sulfur.
spray:Perform before each use of the sterile room. Spray can promote the sedimentation of particles and microorganisms in the air, prevent the dust on the table and the ground, and have a bactericidal effect. Spray with 5% carbolic acid.
Ultraviolet radiation:Perform before each use of the sterile room. Ultraviolet light has a good bactericidal effect. Generally, the UV lamp should be turned on for 30 to 60 minutes.
(three),Clean room work procedure
Sterile room sterilization
★ Apply ultraviolet light for 30 minutes or more before each use, or spray 5% carbolic acid to the inner and outer chambers 30 minutes before use.
Secondary sterilization
★ After washing your hands with soap, move the required equipment into the outer room; replace the sterilized WORK clothes, work caps and work shoes in the outer room, wear a mask, and then dip the hands with 2% phenol soap for 2 minutes.
(IV) Sterile laboratory technical parameters

Description:
1. The wind speed of the air supply port of the locker room and the operation room is ≥0.3 m/s, the wind speed of the air shower room is ≥15 m/s, the static pressure difference between the operation room and the environment is ≥5 Pa, and the system noise is ≤60 dB.
2, the secondary (P2) sterile room is 10,000, local 100, in line with the current universal US federal 209E standard.
3. The secondary sterile room contains high, medium and primary effect filter system, small central air conditioning system, laminar air supply, and well type return air. The dressing room and the air shower are equipped with an automatic body sensing system.
4. In addition to the characteristics of the secondary sterile room, the third-grade (p3) sterile laboratory has also set up electronic interlocking doors, transmission window interlocks, epoxy self-leveling floors, and sewage disinfection facilities.
5. Configure the secondary biological safety cabinet. The laboratory and buffer room are all negative pressure space. The relative pressure between the experiments is -30Pa--40Pa, and the relative pressure between buffers is -15Pa--20Pa.